Đang chuẩn bị liên kết để tải về tài liệu:
Retrovirology Research BioMed Central Open Access A novel HIV-1 restriction factor that is

Retrovirology Research BioMed Central Open Access A novel HIV-1 restriction factor that is biologically distinct from APOBEC3 cytidine deaminases in a human T cell line CEM.NKR Tao Zhou†, Yanxing Han†, Ying Dang†, Xiaojun Wang and Yong-Hui Zheng* Address: Department of Microbiology & Molecular Genetics, Michigan State University, East Lansing, MI 48824-4320, USA Email: Tao Zhou - taoz@msu.edu; Yanxing Han - yanxing@msu.edu; Ying Dang - dandin@msu.edu; Xiaojun Wang - wangxi20@msu.edu; Yong-Hui Zheng* - zhengyo@msu.edu * Corresponding author †Equal contributors Published: 3 April 2009 Retrovirology 2009, 6:31 doi:10.1186/1742-4690-6-31 Received: 12 January 2009 Accepted: 3 April 2009 This article is available from: http://www.retrovirology.com/content/6/1/31 © 2009 Zhou et al; licensee BioMed Central Ltd | Retrovirology BioMed Central Research A novel HIV-I restriction factor that is biologically distinct from APOBEC3 cytidine deaminases in a human T cell line CEM.NKR Tao Zhouf Yanxing Hant Ying Dangt Xiaojun Wang and Yong-Hui Zheng Open Access Address Department of Microbiology Molecular Genetics Michigan State University East Lansing MI 48824-4320 USA Email Tao Zhou - taoz@msu.edu Yanxing Han - yanxing@msu.edu Ying Dang - dandin@msu.edu Xiaojun Wang - wangxi20@msu.edu Yong-Hui Zheng - zhengyo@msu.edu Corresponding author tEqual contributors Published 3 April 2009 Received 12 January 2009 Retrovirology 2009 6 31 doi 10.1186 1742-4690-6-3 1 Accepted 3 April 2009 This article is available from http www.retrovirology.com content 6 1 31 2009 Zhou et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Isolation of novel retroviral restriction factors will open new avenues for anti-HIV AIDS treatment. Although HIV-1 replication is restricted by APOBEC3G APOBEC3F TRIM5a and CD317 none defend HIV-1 infection under natural conditions. Previously we demonstrated a host factor from the human T cell line CEM.NKR that potently restricted wild-type HIV-1 replication. Interestingly this restriction resembled the APOBEC3G APOBEC3F pattern in that viral replication was inhibited from the second round of replication cycle at a post-entry step. Results Here we further characterized this factor and found it distinguishable from the known anti-HIV APOBEC3 proteins. Although CEM.NKR cells expressed both APOBEC3G and APOBEC3F their levels were at least 10 or 4-fold lower than those in H9 cells and importantly Vif effectively neutralized their activity. Among