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Báo cáo y học: " Complementation of diverse HIV-1 Env defects through cooperative subunit interactions: a general property of the functional trimer"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Retrovirology cung cấp cho các bạn kiến thức về ngành y đề tài: " Complementation of diverse HIV-1 Env defects through cooperative subunit interactions: a general property of the functional trimer. | BioMed Central Retrovirology Research Complementation of diverse HIV-I Env defects through cooperative subunit interactions a general property of the functional trimer Karl Salzwedel1 2 and Edward A Berger 1 Open Access Address Laboratory of Viral Diseases National Institute of Allergy and Infectious Diseases National Institutes of Health Bethesda MD 20892 USA and 2Current address Division of AIDS NIAID 6700-B Rockledge Drive Room 4149 Bethesda MD 20892 USA Email Karl Salzwedel - salzwedelkd@niaid.nih.gov Edward A Berger - edward_berger@nih.gov Corresponding author Published II August 2009 Received 4 July 2009 Accepted 1 1 August 2009 Retrovirology 2009 6 75 doi 10.1186 1742-4690-6-75 This article is available from http www.retrovirology.cOm content 6 1 75 2009 Salzwedel and Berger licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The HIV-1 Env glycoprotein mediates virus entry by catalyzing direct fusion between the virion membrane and the target cell plasma membrane. Env is composed of two subunits gp120 which binds to CD4 and the coreceptor and gp41 which is triggered upon coreceptor binding to promote the membrane fusion reaction. Env on the surface of infected cells is a trimer consisting of three gp 1 20 gp4 1 homo-dimeric protomers. An emerging question concerns cooperative interactions between the protomers in the trimer and possible implications for Env function. Results We extended studies on cooperative subunit interactions within the HIV-1 Env trimer using analysis of functional complementation between coexpressed inactive variants harboring different functional deficiencies. In assays of Env-mediated cell fusion complementation was observed between variants with a wide range of defects in both