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Báo cáo y học: " Maleic anhydride-modified chicken ovalbumin as an effective and inexpensive anti-HIV microbicide candidate for prevention of HIV sexual transmission"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Retrovirology cung cấp cho các bạn kiến thức về ngành y đề tài: " Maleic anhydride-modified chicken ovalbumin as an effective and inexpensive anti-HIV microbicide candidate for prevention of HIV sexual transmission. | Li et al. Retrovirology 2010 7 37 http www.retrovirology.eom content 7 1 37 gtr RETR0VIR0L0GY RESEARCH Open Access Maleic anhydride-modified chicken ovalbumin as an effective and inexpensive anti-HIV microbicide candidate for prevention of HIV sexual transmission Lin Li 1 2 Pengyuan Qiao2 Jie Yang1 Lu Lu2 Suiyi Tan1 Hong Lu2 Xiujuan Zhang2 Xi Chen2 Shuguang Wu1 Shibo Jiang 1 2 and Shuwen Liu 1 Abstract Background Previous studies have shown that 3-hydroxyphthalic anhydride HP -modified bovine milk protein p-lactoglobulin P-LG is a promising microbicide candidate. However concerns regarding the potential risk of prion contamination in bovine products and carcinogenic potential of phthalate derivatives were raised. Here we sought to replace bovine protein with an animal protein of non-bovine origin and substitute HP with another anhydride for the development of anti-HIV microbicide for preventing HIV sexual transmission. Results Maleic anhydride ML succinic anhydride SU and HP at different conditions and variable pH values were used for modification of proteins. All the anhydrate-modified globulin-like proteins showed potent anti-HIV activity which is correlated with the percentage of modified lysine and arginine residues in the modified protein. We selected maleic anhydride-modified ovalbumin ML-OVA for further study because OVA is easier to obtain than P-LG and ML is safer than HP. Furthermore ML-OVA exhibited broad antiviral activities against HIV-1 HIV-2 SHIV and SIV. This modified protein has no or low in vitro cytotoxicity to human T cells and vaginal epithelial cells. It is resistant to trypsin hydrolysis possibly because the lysine and arginine residues in OVA are modified by ML. Mechanism studies suggest that ML-OVA inhibits HIV-1 entry by targeting gp120 on HIV-1 virions and also the CD4 receptor on the host cells. Conclusion ML-OVA is a potent HIV fusion entry inhibitor with the potential to be developed as an effective safe and inexpensive anti-HIV .