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Bóa cáo y học: " Early apoptosis of blood monocytes in the septic host: is it a mechanism of protection in the event of septic shock"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care cung cấp cho các bạn kiến thức về ngành y đề tài: Early apoptosis of blood monocytes in the septic host: is it a mechanism of protection in the event of septic shock? | Available online http ccforum.eom content 10 3 R76 Research Early apoptosis of blood monocytes in the septic host is it a mechanism of protection in the event of septic shock Evangelos J Giamarellos-Bourboulis1 Christina Routsi2 Diamantis Plachouras1 Vassiliki Markaki2 Maria Raftogiannis1 Dimitrios Zervakis2 Vassilios Koussoulas1 Stylianos Orfanos3 Anastasia Kotanidou2 Apostolos Armaganidis3 Charis Roussos2 and Helen Giamarellou1 Open Access 14th Department of Internal Medicine University of Athens Medical School Greece 21 st Department of Critical Care University of Athens Medical School Greece 32nd Department of Critical Care University of Athens Medical School Greece Corresponding author Evangelos J Giamarellos-Bourboulis giamarel@ath.forthnet.gr Received 2 Feb 2006 Revisions requested 22 Feb 2006 Revisions received 28 Mar 2006 Accepted 18 Apr 2006 Published 12 May 2006 Critical Care 2006 10 R76 doi 10.1186 cc4921 This article is online at http ccforum.com content 10 3 R76 2006 Giamarellos-Bourboulis et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Introduction Based on the central role of the triggering of monocytes for the initiation of the septic cascade it was investigated whether apoptosis of blood monocytes in septic patients is connected to their final outcome. Methods Blood monocytes were isolated from 90 patients with septic syndrome due to ventilator-associated pneumonia on days 1 3 5 and 7 from the initiation of symptoms. Apoptosis was defined after incubation with annexin-V-fluorescein isothiocyanate and propidium iodine and reading by a flow cytometer. The function of first-day monocytes was evaluated from the concentrations of tumour necrosis factor alpha TNFa and IL-6 in supernatants of cell