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Báo cáo y học: "Transcriptome analysis of monocyte-HIV interaction"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Transcriptome analysis of monocyte-HIV interactions. | Van den Bergh et al. Retrovirology 2010 7 53 http www.retrovirology.eom content 7 1 53 RETR0VIR0L0GY RESEARCH Open Access Transcriptome analysis of monocyte-HIV interactions D f z l r zd z r D L ZI I 2E r I r E I z rz r Z z 3 E r I lz í I I z Z t Z k3 I z D z z Z xí- z r 4 I z r lr 4 Erl í I_I Z i I it Pk I r 5 6 Rafael van den Deign Eric Florence triKa vnegne iom Boonefaes Johan Grooten Erica noumuys Huyen Thi Thanh Tran1 2 Youssef Gali7 Patrick De Baetselier1 2 Guido Vanham7 8 Geert Raes1 2 Abstract Background During HIV infection and or antiretroviral therapy ART monocytes and macrophages exhibit a wide range of dysfunctions which contribute significantly to HIV pathogenesis and therapy-associated complications. Nevertheless the molecular components which contribute to these dysfunctions remain elusive. We therefore applied a parallel approach of genome-wide microarray analysis and focused gene expression profiling on monocytes from patients in different stages of HIV infection and or ART to further characterise these dysfunctions. Results Processes involved in apoptosis cell cycle lipid metabolism proteasome function protein trafficking and transcriptional regulation were identified as areas of monocyte dysfunction during HIV infection. Individual genes potentially contributing to these monocyte dysfunctions included several novel factors. One of these is the adipocytoKine NAMPT visfatin which we show to be capable of inhibiting HIV at an early step in its life cycle. Roughly half of all genes identified were restored to control levels under ART while the others represented a persistent dysregulation. Additionally several candidate biomarKers in particular CCL1 and CYP2C19 for the development of the abacavir hypersensitivity reaction were suggested. Conclusions Previously described areas of monocyte dysfunction during HIV infection were confirmed and novel themes were identified. Furthermore individual genes associated with these dysfunctions and with .