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Báo cáo y học: "HIV-1 subtype distribution in the Gambia and the significant presence of CRF49_cpx, a novel circulating recombinant form"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: HIV-1 subtype distribution in the Gambia and the significant presence of CRF49_cpx, a novel circulating recombinant form. | de Silva et al. Retrovirology 2010 7 82 http www.retrovirology.eom content 7 1 82 RETR0VIR0L0GY RESEARCH Open Access HIV-1 subtype distribution in the Gambia and the significant presence of CRF49_cpx a novel circulating recombinant form 1.2 1 2 1 s I - - 1 Z-I 1 Thushan I de Silva Roxanne Turner Stéphane Hué Roochi Trikha Carla van Tienen Clayton Onyango 14 1 3 1 Assan Jaye Brian Foley Hilton Whittle Sarah L Rowland-Jones Matthew Cotten Abstract Background Detailed local HIV-1 sequence data are essential for monitoring the HIV epidemic for maintaining sensitive sequence-based diagnostics and to aid in designing vaccines. Results Reported here are full envelope sequences derived from 38 randomly selected HIV-1 infections identified at a Gambian clinic between 1991 and 2009. Special care was taken to generate sequences from circulating viral RNA as uncloned products either by limiting dilution or single genome amplification polymerase chain reaction PCR . Within these 38 isolates eight were subtyped as A and 18 as CRF02_AG. A small number of subtype B C D viruses were identified. Surprising however was the identification of six isolates with subtype J-like envelopes a subtype found normally in Central Africa and the Democratic Republic of the Congo DRC with gag p24 regions that clustered with subtype A sequences. Near full-length sequence from three of these isolates confirmed that these represent a novel circulating recombinant form of HIV-1 now named CRF49_cpx. Conclusions This study expands the HIV-1 sequence database from the Gambia and will provide important data for HIV diagnostics patient care and vaccine development. Background Current data on the HIV epidemic in the Gambia are lacking. The most recent published data on HIV prevalence in the general population are from a nationwide perinatal clinic survey in 2000-2001 and indicate a low but possibly increasing prevalence of HIV-1 infection in the country 1 . More recent data from the Medical Research Council