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Báo cáo y học: " Integrase and integration: biochemical activities of HIV-1 integrase"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Integrase and integration: biochemical activities of HIV-1 integrase. | Retrovirology BioMed Central Open Access Review Integrase and integration biochemical activities of HIV-1 integrase Olivier Delelis 1 Kevin Carayon1 Ali Sa ib2 Eric Deprez1 and Jean- Francois Mouscadet1 Address 1LBPA CNRS Ecole Normale Supérieure de Cachan 61 Avenue du President Wilson 94235 Cachan France and 2CNRS Hôpital SaintLouis 1 Avenue Claude Vellefaux 75475 Paris Cedex 10 France Email Olivier Delelis - delelis@lbpa.ens-cachan.fr Kevin Carayon - carayon@lbpa.ens-cachan.fr Ali Saib - ali.saib@univ-paris-diderot.fr Eric Deprez - deprez@lbpa.ens-cachan.fr Jean-Franẹois Mouscadet - mouscadet@lbpa.ens-cachan.fr Corresponding author Published 17 December 2008 Received II September 2008 Accepted 17 December 2008 Retrovirology 2008 5 114 doi 10.1186 1742-4690-5- 114 This article is available from http www.retrovirology.cOm content 5 1 1 14 2008 Delelis et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Integration of retroviral DNA is an obligatory step of retrovirus replication because proviral DNA is the template for productive infection. Integrase a retroviral enzyme catalyses integration. The process of integration can be divided into two sequential reactions. The first one named 3 -processing corresponds to a specific endonucleolytic reaction which prepares the viral DNA extremities to be competent for the subsequent covalent insertion named strand transfer into the host cell genome by a trans-esterification reaction. Recently a novel specific activity of the full length integrase was reported in vitro by our group for two retroviral integrases HIV-1 and PFV-1 . This activity of internal cleavage occurs at a specific palindromic sequence mimicking the LTR-LTR junction described into the 2-LTR circles which .