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Báo cáo y học: " A real-time view of the TAR:Tat:P-TEFb complex at HIV-1 transcription sites"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " A real-time view of the TAR:Tat:P-TEFb complex at HIV-1 transcription sites. | Retrovirology BioMed Central Short report Open Access A real-time view of the TAR Tat P-TEFb complex at HIV-1 transcription sites Dorothée Molle1 Paolo Maiuri2 Stephanie Boireau1 Edouard Bertrand1 Anna Knezevich2 Alessandro Marcello12 and Eugenia Basyuk 11 Address 1IGMM-CNRS UMR 5535 1919 route de Mende 34293 Montpellier France and laboratory of Molecular Virology ICGEB Padriciano 99 34012 Trieste Italy Email Dorothée Molle - dorothee.molle@igmm.cnrs.fr Paolo Maiuri - maiuri@icgeb.org Stephanie Boireau - stephanie.boireau@igmm.cnrs.fr Edouard Bertrand - edouard.bertrand@igmm.cnrs.fr Anna Knezevich - knezevich@icgeb.org Alessandro Marcello - marcello@icgeb.org Eugenia Basyuk - eugenia.basyuk@igmm.cnrs.fr Corresponding author fEqual contributors Published 30 May 2007 Received 4 May 2007 Retrovirology 2007 4 36 doi 10.1186 1742-4690-4-36 Accepted 30 May 2007 This article is available from http www.retrovirology.cOm content 4 1 36 2007 Molle et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract HIV-1 transcription is tightly regulated silent in long-term latency and highly active in acutely-infected cells. Transcription is activated by the viral protein Tat which recruits the elongation factor P-TEFb by binding the TAR sequence present in nascent HIV-1 RNAs. In this study we analyzed the dynamic of the TAR Tat P-TEFb complex in living cells by performing FRAP experiments at HIV-1 transcription sites. Our results indicate that a large fraction of Tat present at these sites is recruited by Cyclin T1. We found that in the presence of Tat Cdk9 remained bound to nascent HIV-1 RNAs for 71s. In contrast when transcription was activated by PMA