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Báo cáo y học: " 5' long terminal repeat (LTR)-selective methylation of latently infected HIV-1 provirus that is demethylated by reactivation signals"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: 5' long terminal repeat (LTR)-selective methylation of latently infected HIV-1 provirus that is demethylated by reactivation signals. | Retrovirology BioMed Central Open Access Short report 5 long terminal repeat LTR -selective methylation of latently infected HIV-1 provirus that is demethylated by reactivation signals Takaomi Ishida Akiko Hamano Tsukasa Koiwa and Toshiki Watanabe Address Laboratory of Tumor Cell Biology Department of Medical Genome Sciences Graduate School of Frontier Sciences The University of Tokyo 4-6-1 Shirokanedai Minato-ku Tokyo 108-8639 Japan Email Takaomi Ishida - tishida@ims.u-tokyo.ac.jp Akiko Hamano - ahamano@mbox.co.jp Tsukasa Koiwa - koiwa@mac.com Toshiki Watanabe - tnabe@ims.u-tokyo.ac.jp Corresponding author Published 12 October 2006 Received 15 November 2005 Accepted 12 October 2006 Retrovirology 2006 3 69 doi l0.ll86 l 742-4690-3-69 This article is available from http www.retrovirology.com content 3 1 69 2006 Ishida et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract We previously described selective hypermethylation of the 5 -long terminal repeat LTR of HTLV-1 provirus in vivo and in vitro. This prompted us to analyze CpG methylation of the two LTRs of the HIV provirus in chronically infected cell lines. The results demonstrate selective hypermethylation of the 5 LTR of the HIV provirus in ACH-2 cells. Moreover induction of viral gene expression by TNF-a resulted in demethylation of the 5 -LTR. These results suggest that selective epigenetic modification of the 5 LTR of the HIV-1 provirus may be an important mechanism by which proviral activity is suppressed. Findings With the use of highly active anti-retroviral therapy HAART for HIV-infected individuals greater control of viral replication is now possible. The widespread use of HAART has led to a substantial decline in the incidence of acquired .