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Báo cáo y học: " Dicistronic MLV-retroviral vectors transduce neural precursors in vivo and co-express two genes in their differentiated neuronal progeny"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Dicistronic MLV-retroviral vectors transduce neural precursors in vivo and co-express two genes in their differentiated neuronal progeny | Retrovirology BioMed Central Research Open Access Dicistronic MLV-retroviral vectors transduce neural precursors in vivo and co-express two genes in their differentiated neuronal progeny Edmund A Derrington1 Marcelo López-Lastra2 and Jean-Luc Darlix 1 Address 1LaboRétro INSERM U412 Ecole Normale Supérieure de Lyon 46 Allée d Italie Lyon 69364 Cedex 07 France and 2Laboratorio de Virología Molecular Centro de Investigaciones Médicas Pontificia Universidad Católica de Chile Marcoleta 391 Santiago Chile Email Edmund A Derrington - derrington@cgmc.univ-lyon1.fr Marcelo López-Lastra - malopez@med.puc.cl Jean-Luc Darlix - jldarlix@ens-lyon.fr Corresponding author Published 29 September 2005 Received 07 July 2005 Accepted 29 September 2005 Retrovirology 2005 2 60 doi 10.1186 1742-4690-2-60 This article is available from http www.retrovirology.com content 2 1 60 2005 Derrington et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.Org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Dicistronic MLV-based retroviral vectors in which two IRESes independently initiate the translation of two proteins from a single RNA have been shown to direct co-expression of proteins in several cell culture systems. Here we report that these dicistronic retroviral vectors can drive co-expression of two gene products in brain cells in vivo. Injection of retroviral vector producer cells leads to the transduction of proliferating precursors in the external granular layer of the cerebellum and throughout the ventricular regions. Differentiated neurons co-expressing both transgenes were observed in the cerebellum and in lower numbers in distant brain regions such as the cortex. Thus we describe an eukaryotic dicistronic vector system that is capable of transducing mouse neural precursors in vivo and