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Báo cáo y học: "A global view of gene expression in lithium and zinc treated sea urchin embryos: new components of gene regulatory network"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: A global view of gene expression in lithium and zinc treated sea urchin embryos: new components of gene regulatory networks. | Open Access A global view of gene expression in lithium and zinc treated sea urchin embryos new components of gene regulatory networks Albert J Poustka Alexander Kuhn Detlef Groth Vesna Weise Shunsuke Yaguchi A Robert D Burke Ralf Herwig Hans Lehrach and Georgia Panopoulou Addresses Max-Planck Institut fur Molekulare Genetik Evolution and Development Group Ihnestrasse 73 14195 Berlin Germany. University of Victoria Departments of Biology and Biochemistry Microbiology 3800 Finnerty Road Victoria British Columbia Canada V8P 5C5. US National Institutes of Health National Institute of Dental and Craniofacial Research 30 Convent Drive MSC 4326 Bethesda. Maryland 20815 USA. Correspondence Albert J Poustka. Email poustka@molgen.mpg.de Published 16 May 2007 Genome Biology 2007 8 R85 doi 10.1 186 gb-2007-8-5-r85 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2007 8 5 R85 Received 15 January 2007 Revised 12 April 2007 Accepted 16 May 2007 2007 Poustka et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The genome of the sea urchin Strongylocentrotus purpuratus has recently been sequenced because it is a major model system for the study of gene regulatory networks. Embryonic expression patterns for most genes are unknown however. Results Using large-scale screens on arrays carrying 50 to 70 of all genes we identified novel territory-specific markers. Our strategy was based on computational selection of genes that are differentially expressed in lithium-treated embryos which form excess endomesoderm and in zinc-treated embryos in which endomesoderm specification is blocked. Whole-mount in situ hybridization WISH analysis of 700 genes indicates that the