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Báo cáo y học: "A catalog of human cDNA expression clones and its application to structural genomics"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: A catalog of human cDNA expression clones and its application to structural genomics. | Method Open Access A catalog of human cDNA expression clones and its application to structural genomics Konrad Bussow Claudia Quedenau Volker Sievert Janett Tischer Christoph Scheich Harald Seitz Brigitte Hieke Frank H Niesen Frank Gotz Ulrich Harttig and Hans Lehrach Addresses Protein Structure Factory Heubnerweg 6 14059 Berlin Germany. tMax Planck Institute for Molecular Genetics Ihnestrabe 73 14195 Berlin Germany. institute of Medical Physics and Biophysics Charité Medical School Ziegelstrabe 5 9 10117 Berlin Germany. Alpha Bioverfahrenstechnik GmbH Heinrich-Hertz-Strabe 1b 14532 Kleinmachnow Germany. RZPD German Resource Center for Genome Research GmbH Heubnerweg 6 14059 Berlin Germany. Correspondence Konrad Bussow. E-mail buessow@molgen.mpg.de Published 17 August 2004 Genome Biology 2004 5 R71 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2004 5 9 R71 Received 16 April 2004 Revised 21 July 2004 Accepted 23 July 2004 2004 Bussow et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli. A cDNA expression library of 10 825 clones was screened by small-scale expression and purification and 2 746 clones were identified. Sequence and protein-expression data were entered into a public database. A set of 163 clones was selected for structural analysis and 17 proteins were prepared for crystallization leading to three new structures. Background Structural genomics and structural proteomics involve the systematic structural analysis of large sets of proteins 1 2 . Structural analysis requires protein