Đang chuẩn bị liên kết để tải về tài liệu:
Báo cáo y học: "Transposon technology and vertebrate functional genomics"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Transposon technology and vertebrate functional genomics. | Meeting report Transposon technology and vertebrate functional genomics Wenfeng An and Jef D Boeke Address Department of Molecular Biology and Genetics and High Throughput Biology Center Johns Hopkins University School of Medicine Baltimore MD 21205 USA. Correspondence Jef D Boeke. E-mail jboeke@jhmi.edu Published 2 December 2005 Genome Biology 2005 6 361 doi l0.ll86 gb-2005-6-l2-36l The electronic version of this article is the complete one and can be found online at http genomebiology.com 2005 6 l2 36l 2005 BioMed Central Ltd A report on the Third Annual International Conference on Transposition and Animal Biotechnology Minneapolis USA 2324 June 2005 and the FASEB Summer Research Conference Mammalian Mobile Elements Tuscon USA 4-9 June 2005. Transposons are mobile genetic elements with the ability to move to new sites in host genomes. This mobility gives them awesome potential as genome-altering tools for somatic and germline mutagenesis and as gene-delivery tools in the laboratory and for gene therapy. Two meetings on transposons this summer in Minneapolis and Tucson revealed the impressive progress in this field with emphasis on transposons in vertebrates. The following report describes a few of the highlights from these meetings. Transposable elements are classified as either DNA transposons or retrotransposons on the basis of their mode of transposition. Eukaryotic DNA transposons transpose by a conservative cut-and-paste mechanism this group includes the Tc1 mariner hAT P-element piggyBac Mutator and En Spm families. Retrotransposons replicate via an RNA intermediate by a copy-and-paste mechanism and are further subdivided into long terminal repeat LTR - and non-LTR types. LTR-retrotransposons are widely distributed among diverse eukaryotes. Phylogenetic analyses based on reverse transcriptase indicate the existence of at least four distinct lineages of LTR-retrotransposons and five groups of non-LTR retrotransposons. The list is expanding as more organisms