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Báo cáo sinh học: "Tissue distribution of a plasmid DNA encoding Hsp65 gene is dependent on the dose administered through intramuscular delivery"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Tissue distribution of a plasmid DNA encoding Hsp65 gene is dependent on the dose administered through intramuscular delivery | Genetic Vaccines and Therapy BioMed Central Research Open Access Tissue distribution of a plasmid DNA encoding Hsp65 gene is dependent on the dose administered through intramuscular delivery AAM Coelho-Castelo1 2 AP Trombone1 2 RS Rosada1 2 RR Santos Jr1 2 VLD Bonato1 2 A Sartori3 and CL Silva 1 2 Address 1Departamento de Bioquímica e Imunologia Faculdade de Medicina Universidade de São Paulo Ribeirão Preto SP Brazil 2REDE-TB Rede Brasileira de combate à tuberculose USP Riberiao Preto São Paulo Brasil and 3Instituto de Biociências UNESP Botucatu São Paulo Brasil Email AAM Coelho-Castelo - arlete@fmrp.usp.br APTrombone - apfavaro@rpm.fmrp.usp.br RS Rosada- rogerio@hotmail.com RR Santos - jrsr@cpt.fmrp.usp.br VLD Bonato - vlbonato@fmrp.usp.br A Sartori - sartori@ibb.unesp.br CL Silva - Clsiva@fmrp.usp.br Corresponding author Published 30 January 2006 Received 14 October 2005 z-. .JTÌ AAA Al J_. IA I Irtz I A-m M-I-Z A I Accepted 30 January 2006 Genetic Vaccines and Therapy 2006 4 1 doi l0.ll86 l479-0556-4-l This article is available from http www.gvt-journal.cOm content 4 l l 2006 Coelho-Castelo et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract In order to assess a new strategy of DNA vaccine for a more complete understanding of its action in immune response it is important to determine the in vivo biodistribution fate and antigen expression. In previous studies our group focused on the prophylactic and therapeutic use of a plasmid DNA encoding the Mycobacterium leprae 65-kDa heat shock protein Hsp65 and achieved an efficient immune response induction as well as protection against virulent M. tuberculosis challenge. In the present study we examined in vivo tissue distribution of naked DNA-Hsp65 vaccine the Hsp65