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Báo cáo sinh học: " Molecular cloning and partial sequence characterization of the duplicated amylase genes from Drosophila erecta"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Molecular cloning and partial sequence characterization of the duplicated amylase genes from Drosophila erecta | Genet Sei Evol 1990 22 57-64 Elsevier INRA 57 Original article Molecular cloning and partial sequence characterization of the duplicated amylase genes from Drosophila erecta L Bally-Cuif V Payant s Abukashawa BF Benkel DA Hickey Department of Biology University of Ottawa Ottawa Ontario KIN 6N5 Canada Received 16 May 1989 accepted 15 November 1989 Summary - Previous studies have shown that the a-amylase gene is duplicated in each of the 8 species comprising the Drosophila melanogaster specie subgroup. In this study a genomic library in phage lambda was prepared from the DNA of 1 species member of this subgroup D erecta. This gene library was screened with an amylase-specific probe from the closely-related species D melanogaster One of the cross-hybridizing phage clones was isolated insert fragments were sub-cloned into plasmid vectors and the identity of the cloned locus was verified by DNA sequencing. The results confirm that this species contains 2 very similar copies of the amylase-coding sequence as does D melanogaster. Within the amylase-coding region there is a 3 sequence divergence between the 2 species this value rises to 6 if we consider only silent sites within the coding region. Sequence divergence between D erecta and D melanogaster in the non-coding intergenic region is approximately 15 . These results constitute the first molecular sequence data available for protein-coding genes of D erecta. The data set allows us to calculate an estimated divergence time of more than 10 million years between the 2 species. This is consistent with the results of previous phylogenetic studies. Drosophila erecta I a-amylase molecular evolution gene conversion Resume Clonage et caractérisation d une sequence partielle des gènes dupliqués de 1 amylase chez Drosophila erecta - Des etudes antérieures ont montré que le gène a-amylase étaự dupliqụé chez chacune des 8 espèces du sous-groupe Drosophila melanogaster. Dans la présẹnte etude une banque génọmique a été préparée .