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Báo cáo y học: "Deciphering the molecular machinery of stem cells: a look at the neoblast gene expression profile"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Deciphering the molecular machinery of stem cells: a look at the neoblast gene expression profile. | Open Access Deciphering the molecular machinery of stem cells a look at the neoblast gene expression profile Leonardo Rossi Alessandra Salvetti Francesco M Marincola Annalisa Lena Paolo Deri Linda Mannini Renata Batistoni Ena Wang and Vittorio Gremigni Addresses Dipartimento di Morfologia Umana e Biologia Applicata Sezione di Biologia e Genetica Università di Pisa Via Volta Pisa 56126 Italy. Department of Transfusion Medicine Warren G Magnuson Clinical Center National Institutes of Health Central Drive Bethesda Maryland 20892 USA. Dipartimento di Biologia Unità di Biologia Cellulare e dello Sviluppo Università di Pisa Via Carducci Pisa 56010 Italy. Correspondence Leonardo Rossi. Email leoros@biomed.unipi.it Published 20 April 2007 Genome Biology 2007 8 R62 doi 10.1 186 gb-2007-8-4-r62 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2007 8 4 R62 Received 24 January 2007 Revised 23 March 2007 Accepted 20 April 2007 2007 Rossi et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Mammalian stem cells are difficult to access experimentally model systems that can regenerate offer an alternative way to characterize stem cell related genes. Planarian regeneration depends on adult pluripotent stem cells - the neoblasts. These cells can be selectively destroyed using X-rays enabling comparison of organisms lacking stem cells with wild-type worms. Results Using a genomic approach we produced an oligonucleotide microarray chip the Dj600 chip which was designed using selected planarian gene sequences. Using this chip we compared planarians treated with high doses of X-rays which eliminates all neoblasts with wild-type worms which led to identification of