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Báo cáo y học: "Whole genome sequencing of a single Bos taurus animal for single nucleotide polymorphism discovery"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Whole genome sequencing of a single Bos taurus animal for single nucleotide polymorphism discovery. | Open Access Researc h Whole genome sequencing of a single Bos taurus animal for single nucleotide polymorphism discovery Sebastian H EckH Anna Benet-PagèsH Krzysztof Flisikowskf Thomas Meitinger Ruedi Fries and Tim M Strom Addresses institute of Human Genetics Helmholtz Zentrum Munchen German Research Center for Environmental Health Ingolstadter Landstr. 85764 Neuherberg Germany. Tehrstuhl fur Tierzucht Technische Universitat Munchen Hochfeldweg 85354 Freising-Weihenstephan Germany. institute of Human Genetics Klinikum rechts der Isar Technische Universitat Munchen Trogerstr. 81675 Munchen Germany. H These authors contributed equally to this work. Correspondence Tim M Strom. Email TimStrom@helmholtz-muenchen.de Published 6 August 2009 Genome Biology 2009 10 R82 doi l0.ll86 gb-2009- 10-8-r82 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2009 10 8 R82 Received 21 April 2009 Revised 22 June 2009 Accepted 6 August 2009 2009 Eck et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The majority of the 2 million bovine single nucleotide polymorphisms SNPs currently available in dbSNP have been identified in a single breed Hereford cattle during the bovine genome project. In an attempt to evaluate the variance of a second breed we have produced a whole genome sequence at low coverage of a single Fleckvieh bull. Results We generated 24 gigabases of sequence mainly using 36-bp paired-end reads resulting in an average 7.4-fold sequence depth. This coverage was sufficient to identify 2.44 million SNPs 82 of which were previously unknown and 1 1 5 000 small indels. A comparison with the genotypes of the same animal generated on a 50 k oligonucleotide .