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Báo cáo y học: "Genome-wide deletion mutant analysis reveals genes required for respiratory growth, mitochondrial genome maintenance and mitochondrial protein synthesis in Saccharomyces cerevisia"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Genome-wide deletion mutant analysis reveals genes required for respiratory growth, mitochondrial genome maintenance and mitochondrial protein synthesis in Saccharomyces cerevisiae. | Open Access Researc h Genome-wide deletion mutant analysis reveals genes required for respiratory growth mitochondrial genome maintenance and mitochondrial protein synthesis in Saccharomyces cerevisiae Sandra Merz and Benedikt Westermann Addresses Institut fur Zellbiologie Universitat Bayreuth UniversitatsstraBe 30 95440 Bayreuth Germany. tBayreuther Zentrum fur Molekulare Biowissenschaften BZMB Universitat Bayreuth UniversitatsstraBe 30 95440 Bayreuth Germany. Correspondence Benedikt Westermann. Email benedikt.westermann@uni-bayreuth.de Published 14 September 2009 Received 27 July 2009 Genome Biology 2009 10 R95 doi 10.1186 gb-2009-10-9-r95 Accepted I4 September 2009 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2009 10 9 R95 2009 Merz and Westermann licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The mitochondrial respiratory chain produces metabolic energy by oxidative phosphorylation. Biogenesis of the respiratory chain requires the coordinated expression of two genomes the nuclear genome encoding the vast majority of mitochondrial proteins and the mitochondrial genome encoding a handful of mitochondrial proteins. The understanding of the molecular processes contributing to respiratory chain assembly and maintenance requires the systematic identification and functional analysis of the genes involved. Results We pursued a systematic genome-wide approach to define the sets of genes required for respiratory activity and maintenance and expression of the mitochondrial genome in yeast. By comparative gene deletion analysis we found an unexpected phenotypic plasticity among respiratory-deficient mutants and we identified ten previously .