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Báo cáo y học: "Serum-dependent transcriptional networks identify distinct functional roles for H-Ras and N-Ras during initial stages of the cell cycle"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Serum-dependent transcriptional networks identify distinct functional roles for H-Ras and N-Ras during initial stages of the cell cycle. | Open Access Serum-dependent transcriptional networks identify distinct functional roles for H-Ras and N-Ras during initial stages of the cell cycle Esther Castellano Carmen Guerrero Alejandro Nunez Javier De Las Rivas and Eugenio Santos Addresses Centro de Investigación del Cáncer IBMCC CSIC-USAL University of Salamanca Campus Unamuno 37007 Salamanca Spain. Current address Signal Transduction Laboratory Cancer Research UK London Research Institute 44 Lincoln s Inn Fields London WC2A 3PX UK. Correspondence Eugenio Santos. Email esantos@usal.es Published 6 November 2009 Genome Biology 2009 10 RI23 doi 10.1186 gb-2009-I0-II-rI 23 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2009 10 11 R123 Received 2 July 2009 Accepted 6 November 2009 2009 Castellano et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Using oligonucleotide microarrays we compared transcriptional profiles corresponding to the initial cell cycle stages of mouse fibroblasts lacking the small GTPases H-Ras and or N-Ras with those of matching wild-type controls. Results Serum-starved wild-type and knockout ras fibroblasts had very similar transcriptional profiles indicating that H-Ras and N-Ras do not significantly control transcriptional responses to serum deprivation stress. In contrast genomic disruption of H-ras or N-ras individually or in combination determined specific differential gene expression profiles in response to poststarvation stimulation with serum for I hour G0 GI transition or 8 hours mid-GI progression . The absence of N-Ras caused significantly higher changes than the absence of H-Ras in the wave of transcriptional activation linked to G0 GI transition. In .