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Báo cáo y học: "Candidate genes for alcohol preference identified by expression profiling in alcohol-preferring and -nonpreferring reciprocal congenic rats"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Candidate genes for alcohol preference identified by expression profiling in alcohol-preferring and -nonpreferring reciprocal congenic rats . | Liang et al. Genome Biology 2010 11 R11 http genomebiology.eom 2010 11 2 R11 Genome Biology RESEARCH Open Access Candidate genes for alcohol preference identified by expression profiling in aleohol-preferring and -nonpreferring reciprocal eongenie rats Tiebing Liang1 Mark W Kimpel2 Jeanette N McClintick3 Ashley R Skillman1 Kevin McCall4 Howard J Edenberg3 Lucinda G Carr1 Abstract Background Selectively bred alcohol-preferring P and alcohol-nonpreferring NP rats differ greatly in alcohol preference in part due to a highly significant quantitative trait locus QTL on chromosome 4. Alcohol consumption scores of reciprocal chromosome 4 congenic strains NP.P and P.NP correlated with the introgressed interval. The goal of this study was to identify candidate genes that may influence alcohol consumption by comparing gene expression in five brain regions of alcohol-naive inbred alcohol-preferring and P.NP congenic rats amygdala nucleus accumbens hippocampus caudate putamen and frontal cortex. Results Within the QTL region 104 cis-regulated probe sets were differentially expressed in more than one region and an additional 53 were differentially expressed in a single region. Fewer trans-regulated probe sets were detected and most differed in only one region. Analysis of the average expression values across the 5 brain regions yielded 141 differentially expressed cis-regulated probe sets and 206 trans-regulated probe sets. Comparing the present results from inbred alcohol-preferring vs. congenic P.NP rats to earlier results from the reciprocal congenic NP.P vs. inbred alcohol-nonpreferring rats demonstrated that 74 cis-regulated probe sets were differentially expressed in the same direction and with a consistent magnitude of difference in at least one brain region. Conclusions Cis-regulated candidate genes for alcohol consumption that lie within the chromosome 4 QTL were identified and confirmed by consistent results in two independent experiments with reciprocal congenic .