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Báo cáo y học: " b-Lapachone induces heart morphogenetic and functional defects by promoting the death of erythrocytes and the endocardium in zebrafish embryo"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: b-Lapachone induces heart morphogenetic and functional defects by promoting the death of erythrocytes and the endocardium in zebrafish embryos. | Wu et al. Journal of Biomedical Science 2011 18 70 http www.jbiomedsci.eom content 18 1 70 NSC The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access b-Lapachone induces heart morphogenetic and functional defects by promoting the death of erythrocytes and the endocardium in zebrafish embryos 11 2 3 3 4 5 Yi-Ting Wu Che Yi Lin Ming-Yuan Tsai Yi-Hua Chen Yu-Fen Lu Chang-Jen Huang Chao-Min Cheng and Sheng-Ping L Hwang1 3 Abstract Background b-Lapachone has antitumor and wound healing-promoting activities. To address the potential influences of various chemicals on heart development of zebrafish embryos we previously treated zebrafish embryos with chemicals from a Sigma LOPAC1280 library and found several chemicals including b-lapachone that affected heart morphogenesis. In this study we further evaluated the effects of b-lapachone on zebrafish embryonic heart development. Methods Embryos were treated with b-lapachone or dimethyl sulfoxide DMSO at 24 or 48 hours post fertilization hpf for 4 h at 28 C. Heart looping and valve development was analyzed by whole-mount in situ hybridization and histological analysis. For fractional shortening and wall shear stress analyses AB and Tg gatal DsRed embryos were recorded for their heart pumping and blood cell circulations via time-lapse fluorescence microscopy. Dextran rhodamine dye injection into the tail reticular cells was used to analyze circulation. Reactive oxygen species ROS was analyzed by incubating embryos in 5- and 6- -chloromethyl-2 7 -dichloro-dihydrofluorescein diacetate CM-H2DCFDA and recorded using fluorescence microscopy. o-Dianisidine ODA staining and whole mount in situ hybridization were used to analyze erythrocytes. TUNEL assay was used to examine DNA fragmentation. Results We observed a linear arrangement of the ventricle and atrium bradycardia arrhythmia reduced fractional shortening circulation with a few