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Báo cáo y học: "EGb761 protects motoneurons against avulsion-induced oxidative stress in rats Research article"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: EGb761 protects motoneurons against avulsion-induced oxidative stress in rats Research article. | Cheng et al. Journal of Brachial Plexus and Peripheral Nerve Injury 2010 5 12 http www.jbppni.eom content 5 1 12 JOURNAL OF BRACHIAL PLEXUS AND PERIPHERAL NERVE INJURY RESEARCH ARTICLE Open Access EGb761 protects motoneurons against avulsion-induced oxidative stress in rats Xiao Cheng 1 Fo-Lin Liu1 Jun Zhang2 Lin-Lin Wang1 Fang-lan Li1 Shu Liu1 and Li-Hua Zhou 1 Abstract Background Root avulsion of the brachial plexus causes an oxidative stress reaction in the spinal cord and induces dramatic spinal motoneuron death while EGb761 is a natural free radical cleaning agent. This study was designed to investigate the protective effects of intraperitoneally injected EGb761 against neural damage following brachial root avulsion. Methods The effect of EGb761 on avulsion-induced motoneuron injury was studied in 26 total groups of n rats treated as follows. Animals in singular number groups received EGb761 50 mg kg.d and those in complex number groups received normal saline solution i.p. serving as controls. Groups 1-8 were used for the determination of nitric oxide NO levels in the serum and injured spinal cord at the 5 d 2 w 4 w and 6 w time points. Groups 9-16 were used for determination of constitutive nitric oxide synthase cNOS and inducible nitric oxide synthase iNOS levels in injured spinal cord at the 5 d 2 w 4 w and 6 w time points. Groups 17-26 were used for determination of the number of neuronal nitric oxide synthase nNOS -positive and surviving motoneurons in injured C7 ventral horn at the 5 d 2 w 4 w 6 w and 8 w time points. Results Compared to control groups the EGb761 treatment group not only had significant decreased levels of NO in serum at 2 w and 6 w after avulsion but also had reduced levels of NO specifically in the spinal cord at 2 w 4 w and 6 w. The cNOS activity in the spinal cord was also significant decreased at 2 w and 4 w while the iNOS activity in injured C6-T1 spinal segments was reduced at 2 w 4 w and 6 w. All together the percentages of .