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báo cáo khoa học: "Laser ablation-based one-step generation and bio-functionalization of gold nanoparticles conjugated with aptamers"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Laser ablation-based one-step generation and bio-functionalization of gold nanoparticles conjugated with aptamers | Walter et al. Journal of Nanobiotechnology 2010 8 21 http www.jnanobiotechnology.eom content 8 1 21 JOURNAL OF NANOBIOTECHNOLOGY RESEARCH Open Access Laser ablation-based one-step generation and bio-functionalization of gold nanoparticles conjugated with aptamers 12 1 1 2 Johanna G Walter Svea Petersen Frank Stahl Thomas Scheper Stephan Barcikowski Abstract Background Bio-conjugated nanoparticles are important analytical tools with emerging biological and medical applications. In this context in situ conjugation of nanoparticles with biomolecules via laser ablation in an aqueous media is a highly promising one-step method for the production of functional nanoparticles resulting in highly efficient conjugation. Increased yields are required particularly considering the conjugation of cost-intensive biomolecules like RNA aptamers. Results Using a DNA aptamer directed against streptavidin in situ conjugation results in nanoparticles with diameters of approximately 9 nm exhibiting a high aptamer surface density 98 aptamers per nanoparticle and a maximal conjugation efficiency of 40.3 . We have demonstrated the functionality of the aptamer-conjugated nanoparticles using three independent analytical methods including an agglomeration-based colorimetric assay and solid-phase assays proving high aptamer activity. To demonstrate the general applicability of the in situ conjugation of gold nanoparticles with aptamers we have transferred the method to an RNA aptamer directed against prostate-specific membrane antigen PSMA . Successful detection of PSMA in human prostate cancer tissue was achieved utilizing tissue microarrays. Conclusions In comparison to the conventional generation of bio-conjugated gold nanoparticles using chemical synthesis and subsequent bio-functionalization the laser-ablation-based in situ conjugation is a rapid one-step production method. Due to high conjugation efficiency and productivity in situ conjugation can be easily used for high throughput .