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Báo cáo khoa hoc:" Variance in multiplex suspension array assays: carryover of microspheres between sample wells"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Variance in multiplex suspension array assays: carryover of microspheres between sample wells | Journal of Negative Results in BioMedicine BioMed Central Research Open Access Variance in multiplex suspension array assays carryover of microspheres between sample wells Brian Hanley 1 2 Address Microbiology Graduate Group University of California Davis CA 95616 USA and 2BW Education and Forensics 2710 Thomes Avenue Cheyenne Wyoming 82001 USA Email Brian Hanley - bphanley@ucdavis.edu Corresponding author Published 25 April 2007 Received 30 March 2007 Journal of Negative Results in BioMedicine 2007 6 6 doi 10.1186 1477-5751-6-6 Accepted 25Apnl 2007 This article is available from http www.jnrbm.com content 6 1 6 2007 Hanley licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.Org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background This study was undertaken because of the accidental observation that a sample of 60 beads was obtained by the instrument from a completely dry unused well in a 96 well plate. Others have observed unexplained outliers in replicated wells. The problem was first observed on an older instrument and replicated on a new instrument. Methods and results Data is presented from two instruments using a multiple blank following well experiment that shows a surprising amount of carryover that has an unexpected nature. When it occurs it does not necessarily decline from one well to the next. There appears to be two types of carryover one that is small predictable and declines consistently and another which is potentially very large unpredictable and does not decline. The former can be compensated for or ignored. The latter cannot be addressed without using multiple replicated samples or an intraplex method. Conclusion This problem has significance for analysis of results obtained with suspended microarray instruments. A special notation is made that .