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báo cáo khoa học: " Integration of microRNA changes in vivo identifies novel molecular features of muscle insulin resistance in type 2 diabetes"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Integration of microRNA changes in vivo identifies novel molecular features of muscle insulin resistance in type 2 diabetes | Gallagher et al. Genome Medicine 2010 2 9 http genomemedicine.eom content 2 2 9 Genome Medicine RESEARCH Open Access Integration of microRNA changes in vivo identifies novel molecular features of muscle insulin resistance in type 2 diabetes Hin I 2112 Cl h o r1 ismillia 2cHoolo2 3 Pomillo I4ollof1 2 Anclorc p I liolcot i2 II ichl Pomon i4 r hric1 Ì2n p Picchof2 Idii iJGdiidyi lei Ca 11 Iiiidoci leeie reii lilleKellei AI Ideisni Nielsei I judnnei I lei lyi diiibtidiirrisciiei Karim Rodei1 Johi Bdbidj1 Cldes Wdhlestedt5 Gyoigy Hutvdgiei4 Beite K Pedeisei2 did Jdmes A Timmois 1 3 6 7 Abstract Background Skeletdl muscle iisulii resistance IR is coisideied d ciiticdl compoieit of type II didbetes yet to ddte IR hds evdded characterizatioi dt the globdl geie expiessioi level ii humdis. MicioRNAs miRNAs die coisideied fiie-scdle iheostdts of pioteii-codiig geie product dbuiddice. The ieldtive impoitdice did mode of dctioi of miRNAs ii humdi complex disedses iemdiis to be fully eluciddted. We produce d globdl mdp of codiig did ioi-codiig RNAs ii humdi muscle IR with the dim of ideitifyiig iovel disedse biomdikeis. Methods We profiled 47 000 mRNA sequeices did 500 humdi miRNAs usiig geie-chips did 118 subjects i 71 pdtieits veisus i 47 coitiols . A tissue-specific geie-idikiig system wds developed to stidtify thousdids of miRNA tdiget-geies iemoviig fdlse positives yieldiig d weighted iihibitoi scoie which iitegidted the iet impdct of both up- did dowi-ieguldted miRNAs. Both iifoimdtic did pioteii detectioi vdliddtioi wds used to verify the piedictiois of in vivo chdiges. Results The muscle mRNA tidisciiptome is iivdiidit with iespect to iisulii oi glucose homeostdsis. Ii coitidst d thiid of miRNAs detected ii muscle weie dlteied ii disedse i 62 mdiy chdigiig piioi to the oiset of cliiicdl didbetes. The iovel idikiig metiic ideitified six cdioiicdl pdthwdys with piovei liiks to metdbolic disedse while the coitiol ddtd demoistidted io eiiichmeit. The Beijdmiii-Hochbeig .