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báo cáo khoa học:" Microvascular engineering in perfusion culture: immunohistochemistry and CLSM findings"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Microvascular engineering in perfusion culture: immunohistochemistry and CLSM findings | Head Face Medicine BioMed Central Research Microvascular engineering in perfusion culture immunohistochemistry and CLSM findings Bernhard Frerich Kerstin Zuckmantel and Alexander Hemprich Open Access Address Department of Oral and Maxillofacial Surgery Plastic Facial Surgery University of Leipzig Nurnberger Str. 57 D-04103 Leipzig Germany Email Bernhard Frerich - frerich@medizin.uni-leipzig.de Kerstin Zuckmantel - kzueck@web.de Alexander Hemprich - alexander.hemprich@medizin.uni-leipzig.de Corresponding author Published 16 August 2006 Received 03 May 2006 Head Face Medicine 2006 2 26 doi 10.1186 1746-160X-2-26 Accepted 16 August 2006 This article is available from http www.head-face-med.cOm content 2 1 26 2006 Frerich et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background One of the most challenging problems in tissue engineering is the establishment of vascular supply. A possible approach might be the engineering of microvasculature in vitro and the supply by engineered feeder vessels. Methods An in vitro model for a small-diameter vessel was developed and made from adipose tissue stromal cells and human umbilical vein endothelial cells in a tube-like gelatine scaffold. The number of branches emerging from the central lumen and the morphology of the central lumen of the vessel equivalent were assessed after 16 days of either pulsatile perfusion culture or culture in rotating containers by evaluation of immunohistochemically stained sections n 6 pairs of cultures . Intramural capillary network formation was demonstrated in five experiments with confocal laser scanning microscopy. Results Perfused specimens showed a round or oval lumen lined by a single layer of endothelial cells whereas following rotation