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báo cáo khoa học: " Comparative genomic analysis and expression of the APETALA2-like genes from barley, wheat, and barley-wheat amphiploids"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Comparative genomic analysis and expression of the APETALA2-like genes from barley, wheat, and barley-wheat amphiploids | BMC Plant Biology BioMed Central Open Access Research article Comparative genomic analysis and expression of the APETALA2-like genes from barley wheat and barley-wheat amphiploids Javier Gil-Humanes Fernando Pistón Antonio Martín and Francisco Barro Address Departamento de Mejora Genética Vegetal. Institute de Agricultura Sostenible CSIC 14080-Córdoba Spain Email Javier Gil-Humanes - javigil@ias.csic.es Fernando Pistón - b62pipif@uco.es Antonio Martín - ge1mamua@uco.es Francisco Barro - fbarro@ias.csic.es Corresponding author Published 29 May 2009 Received 26 February 2009 BMC Plant Biology 2009 9 66 doi 10.1186 1471-2229-9-66 Accepted 29 May 2009 This article is available from http www.biomedcentral.com 1471-2229 9 66 2009 Gil-Humanes et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.Org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The APETALA2-like genes form a large multi-gene family of transcription factors which play an important role during the plant life cycle being key regulators of many developmental processes. Many studies in Arabidopsis have revealed that the APETALA2 AP2 gene is implicated in the establishment of floral meristem and floral organ identity as well as temporal and spatial regulation of flower homeotic gene expression. Results In this work we have cloned and characterised the AP2-like gene from accessions of Hordeum chilense and Hordeum vulgare wild and domesticated barley respectively and compared with other AP2 homoeologous genes including the Q gene in wheat. The Hordeum AP2-like genes contain two plant-specific DNA binding motifs called AP2 domains as does the Q gene of wheat. We confirm that the H. chilense AP2-like gene is located on chromosome 5Hch. Patterns of expression of the AP2-like genes were examined in floral