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Báo cáo y học: " Monoclonal antibody induced with inactived EV71-Hn2 virus protects mice against lethal EV71-Hn2 virus infection"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Monoclonal antibody induced with inactived EV71-Hn2 virus protects mice against lethal EV71-Hn2 virus infection | Chang et al. Virology Journal 2010 7 106 http www.virologyj.eom content 7 1 106 VIROLOGY JOURNAL RESEARCH Open Access Monoclonal antibody induced with inactived EV71-Hn2 virus protects mice against lethal EV71-Hn2 virus infection Guo-hui Chang Yan-jun Luo Xiao-yan Wu Bing-yin Sif Lei Lin and Qing-yu Zhu Abstract Background Enterovirus 71 EV71 is a viral pathogen that belongs to the Picornaviridaefamily EV71-infected children can develop severe neurological complications leading to rapid clinical deterioration and death. Results In this study several monoclonal antibodies MAbs were produced by immunizing mice with the inactived EV71 Henan Hn2 virus strain. The isolated MAbs were characterised by in vitro neutralizing analysis and peptide ELISA. ELISA assay showed that the neutralizing monoclonal antibody 4E8 specifically reacted with synthetic peptides which contain amino acid 240-250 and 250-260 of EV71 VP1. The in vivo protection assay showed that 4E8 can protect two-day-old BALB c mice against the lethal challenge of EV71 virus. Conclusion The MAb 4E8 could be a promising candidate to be humanized and used for treatment of EV71 infection. Background Enterovirus 71 EV71 is a viral pathogen within the Picornaviridae family and causes clinical diseases in humans with manifestations such as herpangina aseptic meningitis encephalitis pulmonary edema and hand foot and mouth disease HFMD . EV71-infected children can develop severe neurological complications that lead to rapid clinical deterioration and death 1 . Since the first instances in 1969 2 several large epidemics of HFMD have been reported in the Asia-Pacific region especially in Southeast Asia 3-6 . In China between 1999-2009 HFMD outbreaks caused by EV71 have affected more than 500 000 young children and resulted in more than 200 deaths in cities such as Beijing Shenzhen Guangdong 7-9 . In fact after the eradication of the poliovirus 10 EV71 has been regarded as the most important neurotropic enterovirus. .