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báo cáo khoa học: " Silencing of beta-carotene hydroxylase increases total carotenoid and beta-carotene levels in potato tubers"

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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Silencing of beta-carotene hydroxylase increases total carotenoid and beta-carotene levels in potato tubers | BMC Plant Biology BioMed Central Open Access Silencing of beta-carotene hydroxylase increases total carotenoid and beta-carotene levels in potato tubers Gianfranco Diretto1 Ralf Welsch2 Raffaela Tavazza1 Fabienne Mourgues1 Daniele Pizzichini1 Peter Beyer2 and Giovanni Giuliano 1 Address 1ENEA Casaccia Research Center PO Box 2400 Roma 00100AD Italy and 2Faculty for Biology Universitat Freiburg Schanzlestrasse 1 79104 Freiburg Germany Email Gianfranco Diretto - gianfranco.diretto@casaccia.enea.it Ralf Welsch - welschra@web.de Raffaela Tavazza - raffaela.tavazza@casaccia.enea.it Fabienne Mourgues - fabienne.mourgues@trisaia.enea.it Daniele Pizzichini - daniele.pizzichini@casaccia.enea.it Peter Beyer - peter.beyer@biologie.uni-freiburg.de Giovanni Giuliano - giuliano@casaccia.enea.it Corresponding author Published 2 March 2007 Received 17 November 2006 BMC Plant Biology 2007 7 11 doi l0.ll86 l47l-2229-7-ll Accepted 2 March 2007 This article is available from http www.biomedcentral.cOm l47l-2229 7 l l 2007 Diretto et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract_ Background Beta-carotene is the main dietary precursor of vitamin A. Potato tubers contain low levels of carotenoids composed mainly of the xanthophylls lutein in the beta-epsilon branch and violaxanthin in the beta-beta branch . None of these carotenoids have provitamin A activity. We have previously shown that tuber-specific silencing of the first step in the epsilon-beta branch LCY-e redirects metabolic flux towards beta-beta carotenoids increases total carotenoids up to 2.5-fold and beta-carotene up to l4-fold. Results In this work we silenced the non-heme .

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