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báo cáo khoa học: " Identification of novel and candidate miRNAs in rice by high throughput sequencing"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Identification of novel and candidate miRNAs in rice by high throughput sequencing | BMC Plant Biology BioMed Central Research article Identification of novel and candidate miRNAs in rice by high throughput sequencing Ramanjulu Sunkar 1 Xuefeng Zhout2 Yun Zheng2 Weixiong Zhang2 and Jian-Kang Zhu 3 Address Department of Biochemistry and Molecular Biology Oklahoma State University Stillwater OK 74078 USA 2Department of Computer Science and Engineering Washington University in St. Louis 1 Brookings Drive St Louis MO 63130 USA and 3Department of Botany and Plant Sciences University of California Riverside CA 92521 USA Email Ramanjulu Sunkar - rsunkar@biochem.okstate.edu Xuefeng Zhou - xzhou@cse.wustl.edu Yun Zheng - zhengy@cse.wustl.edu Weixiong Zhang - zhang@cse.wustl.edu Jian-Kang Zhu - jian-kang.zhu@ucr.edu Corresponding authors fEqual contributors Open Access Published 29 February 2008 Received 3 January 2008 BMC Plant Biology 2008 8 25 doi 10.1186 1471-2229-8-25 Accepted 29 February 2008 This article is available from http www.biomedcentral.cOm 1471-2229 8 25 2008 Sunkar et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract_ Background Small RNA-guided gene silencing at the transcriptional and post-transcriptional levels has emerged as an important mode of gene regulation in plants and animals. Thus far conventional sequencing of small RNA libraries from rice led to the identification of most of the conserved miRNAs. Deep sequencing of small RNA libraries is an effective approach to uncover rare and lineage- and or species-specific microRNAs miRNAs in any organism. Results In order to identify new miRNAs and possibly abiotic-stress regulated small RNAs in rice three small RNA libraries were constructed from control .