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Báo cáo sinh học: " HIV-1 Vpr activates the G2 checkpoint through manipulation of the ubiquitin proteasome system"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: HIV-1 Vpr activates the G2 checkpoint through manipulation of the ubiquitin proteasome system | Virology Journal BioMed Central Research Open Access HIV-I Vpr activates the G2 checkpoint through manipulation of the ubiquitin proteasome system Jason L DeHart 1 Erik S Zimmerman 1 Orly Ardon1 Carlos MR Monteiro- Filho2 Enrique RArganaraz2 and Vicente Planelles 1 Address 1Division of Cell Biology and Immunology Department of Pathology University of Utah School of Medicine 15 North Medical Drive East 2100 - Room 2520 Salt Lake City UT 84112 USA and 2Laboratório de Farmacologia Molecular CP 04536 Faculdade de Saude Universidade de Brasilia 70919-970 Brasilia DF Brazil Email Jason L DeHart - jason.dehart@path.utah.edu Erik S Zimmerman - erik.zimmerman@path.utah.edu Orly Ardon - orly.ardon@path.utah.edu Carlos MR Monteiro-Filho - carlosmonteirofilho@yahoo.com.br Enrique R Arganaraz - enrique@unb.br Vicente Planelles - vicente.planelles@path.utah.edu Corresponding author fEqual contributors Published 8 June 2007 Received 4 June 2007 Accepted 8 June 2007 Virology Journal 2007 4 57 doi l0.ll86 l 743-422X-4-57 This article is available from http www.virologyj.cOm content 4 1 57 2007 DeHart et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract HIV-1 Vpr is a viral accessory protein that activates ATR through the induction of DNA replication stress. ATR activation results in cell cycle arrest in G2 and induction of apoptosis. In the present study we investigate the role of the ubiquitin proteasome system UPS in the above activity of Vpr. We report that the general function of the UPS is required for Vpr to induce G2 checkpoint activation as incubation of Vpr-expressing cells with proteasome inhibitors abolishes this effect. We further investigated in detail the specific E3 ubiquitin ligase subunits that Vpr manipulates. We