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Báo cáo sinh học: "Concomitant detection of IFNa signature and activated monocyte/dendritic cell precursors in the peripheral blood of IFNa-treated subjects at early times after repeated local cytokine treatments"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Concomitant detection of IFNa signature and activated monocyte/dendritic cell precursors in the peripheral blood of IFNa-treated subjects at early times after repeated local cytokine treatments | Aricò et al. Journal of Translational Medicine 2011 9 67 http www.translational-medicine.eom content 9 1 67 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Concomitant detection of IFNa signature and activated monocyte dendritic cell precursors in the peripheral blood of IFNa-treated subjects at early times after repeated local cytokine treatments 1.2 1.3 1 1 24 2 Eleonora Aricò Luciano Castiello Francesca Urbani Paola Rizza Monica C Panelli Ena Wang Francesco M Marincola2 and Filippo Belardelli1 Abstract Background Interferons alpha IFNa are the cytokines most widely used in clinical medicine for the treatment of cancer and viral infections. Among the immunomodulatory activities possibly involved in their therapeutic efficacy the importance of IFNa effects on dendritic cells DC differentiation and activation has been considered. Despite several studies exploiting microarray technology to characterize IFNa mechanisms of action there is currently no consensus on the core signature of these cytokines in the peripheral blood of IFNa-treated individuals as well as on the existence of blood genomic and proteomic markers of low-dose IFNa administered as a vaccine adjuvant. Methods Gene profiling analysis with microarray was performed on PBMC isolated from melanoma patients and healthy individuals 24 hours after each repeated injection of low-dose IFNa administered as vaccine adjuvant in two separate clinical trials. At the same time points cytofluorimetric analysis was performed on CD14 monocytes to detect the phenotypic modifications exerted by IFNa on antigen presenting cells precursors. Results An IFNa signature was consistently observed in both clinical settings 24 hours after each repeated administration of the cytokine. The observed modulation was transient and did not reach a steady state level refractory to further stimulations. The molecular signature observed ex vivo largely matched the one detected in CD14 monocytes exposed in vitro to IFNa including .