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Báo cáo sinh học: " CODEHOP-mediated PCR – A powerful technique for the identification and characterization of viral genomes"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: CODEHOP-mediated PCR – A powerful technique for the identification and characterization of viral genomes | Virology Journal BioMed Central Review CODEHOP-mediated PCR - A powerful technique for the identification and characterization of viral genomes Timothy M Rose Open Access Address Department of Pathobiology Box 357238 School of Public Health and Community Medicine University of Washington Seattle WA 98195 USA Email TimothyM Rose - trose@u.washington.edu Corresponding author Published 15 March 2005 Received 08 January 2005 Accepted 15 March 2005 Virology Journal 2005 2 20 doi 10.1 186 1743-422X-2-20 This article is available from http www.virologyj.cOm content 2 1 20 2005 Rose licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Consensus-Degenerate Hybrid Oligonucleotide Primer CODEHOP PCR primers derived from amino acid sequence motifs which are highly conserved between members of a protein family have proven to be highly effective in the identification and characterization of distantly related family members. Here the use of the CODEHOP strategy to identify novel viruses and obtain sequence information for phylogenetic characterization gene structure determination and genome analysis is reviewed. While this review describes techniques for the identification of members of the herpesvirus family of DNA viruses the same methodology and approach is applicable to other virus families. Introduction Only a very small fraction of the vast number of viral species belonging to the different virus families have been identified and characterized to date. The majority of these uncharacterized viral species are found in host organisms which have not been targeted in biomedical plant or animal research. However recent reports have noted an increase in the occurrence of viral diseases not only in humans but in animals and plants as .