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Báo cáo hóa học: " Detection of virus mRNA within infected host cells using an isothermal nucleic acid amplification assay: marine cyanophage gene expression within Synechococcus sp"

Tham khảo tài liệu 'báo cáo hóa học: " detection of virus mrna within infected host cells using an isothermal nucleic acid amplification assay: marine cyanophage gene expression within synechococcus sp"', luận văn - báo cáo phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Virology Journal BioMed Central Research Open Access Detection of virus mRNA within infected host cells using an isothermal nucleic acid amplification assay marine cyanophage gene expression within Synechococcus sp Susan D Wharam1 2 3 Matthew J Hall3 and William H Wilson 1 3 4 Address 1Bigelow Laboratory for Ocean Sciences 180 McKown Point West Boothbay Harbor Maine 04575 USA 2Cytocell Ltd. Banbury Business Park Adderbury OX17 3SN UK 3Marine Biological Association Citadel Hill Plymouth PL1 2PB UK and 4Plymouth Marine Laboratory Prospect Place The Hoe Plymouth PL1 3DH UK Email Susan D Wharam - swharam@bigelow.org Matthew J Hall - mjhal@mba.ac.uk William H Wilson - wwilson@bigelow.org Corresponding author Published 6 June 2007 Received 15 March 2007 Accepted 6 June 2007 Virology Journal 2007 4 52 doi 10.1186 1743-422X-4-52 This article is available from http www.virologyj.cOm content 4 1 52 2007 Wharam et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Signal-Mediated Amplification of RNA Technology SMART is an isothermal nucleic acid amplification technology developed for the detection of specific target sequences either RNA for expression or DNA. Cyanophages are viruses that infect cyanobacteria. Marine cyanophages are ubiquitous in the surface layers of the ocean where they infect members of the globally important genus Synechococcus. Results Here we report that the SMART assay allowed us to differentiate between infected and non-infected host cultures. Expression of the cyanophage strain S-PM2 portal vertex gene g20 was detected from infected host Synechococcus sp. WH7803 cells. Using the SMART assay we demonstrated that g20 mRNA peaked 240 - 360 minutes post-infection allowing us to characterise .