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Báo cáo hóa học: " Detection of seroconversion to West Nile virus, Usutu virus and Sindbis virus in UK sentinel chickens"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Detection of seroconversion to West Nile virus, Usutu virus and Sindbis virus in UK sentinel chickens | Virology Journal BioMed Central Open Access Short report Detection of seroconversion to West Nile virus Usutu virus and Sindbis virus in UK sentinel chickens Alan Buckley 1 Alistair Dawson2 and Ernest A Gould1 Address 1CEH Oxford Mansfield Road Oxford OX1 3SR UK and 2CEH Monks Wood Abbots Ripton Huntingdon Cambridgeshire PE28 2LS UK Email Alan Buckley - abuck@ceh.ac.uk Alistair Dawson - asda@ceh.ac.uk Ernest A Gould - eag@ceh.ac.uk Corresponding author Published 04 September 2006 Received 13 July 2006 Accepted 04 September 2006 Virology journal 2006 3 71 doi 10.1186 1743-422X-3-71 This article is available from http www.virologyj.cOm content 3 1 71 2006 Buckley et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Summary_ We previously reported evidence of West Nile virus WNV circulation in UK birds probably introduced by migratory birds from overseas. We now demonstrate WNV-specific seroconversion in sentinel chickens raised on an English farm. Maternal neutralizing antibodies to WNV in hatchlings declined within three weeks. During the following months healthy chickens developed WNV neutralizing antibodies that were confirmed by immunoblotting and indirect immunofluorescence tests using WNV antigens. The proportion of seropositive chickens was higher for WNV than for Usutu virus or Sindbis virus. Attempts to isolate infectious virus or to detect viral RNA in the sera failed. Background West Nile virus WNV and Usutu virus USUV are anti-genically closely related mosquito-borne members of the genus Flavivirus. Sindbis virus SINV is an unrelated mosquito-borne member of the genus Alphavirus. These arthropod-borne viruses arboviruses and many others are known .