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báo cáo hóa học:" Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey | Virology Journal BioMed Central Research Open Access Detection quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR a cross sectional survey Esther AN Aryee 1 Robin L Bailey1 2 Angels Natividad-Sancho2 Steve Kaye1 and Martin J Holland 1 2 Address 1Medical Research Council Laboratories Fajara The Gambia and 2London School of Hygiene and Tropical Medicine London UK Email Esther AN Aryee - earyee@mrc.gm Robin L Bailey - Robin.Bailey@lshtm.ac.uk Angels Natividad-Sancho - Angels.Natividad-Sancho@lshtm.ac.uk Steve Kaye - skaye@mrc.gm Martin J Holland - Martin.Holland@lshtm.ac.uk Corresponding author Published II August 2005 Received 17 June 2005 Accepted 1 1 August 2005 Virology Journal 2005 2 61 doi 10.1186 1743-422X-2-61 This article is available from http www.virologyj.cOm content 2 1 61 2005 Aryee et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Herpes Simplex Virus HSV Genital Ulcer Disease GUD is an important public health problem whose interaction with HIV results in mutually enhancing epidemics. Conventional methods for detecting HSV tend to be slow and insensitive. We designed a rapid PCR-based assay to quantify and type HSV in cervicovaginal lavage CVL fluid of subjects attending a Genito-Urinary Medicine GUM clinic. Vaginal swabs CVL fluid and venous blood were collected. Quantitative detection of HSV was conducted using real time PCR with HSV specific primers and SYBR Green I. Fluorogenic TaqMan Minor Groove Binder MGB probes designed around a single base mismatch in the HSV DNA polymerase I gene were used to type HSV in a separate reaction. The Kalon test was used to detect anti-HSV-2 IgG antibodies in serum. Testing for HIV other Sexually .