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báo cáo hóa học:" Effects of alpha-calcitonin gene-related peptide on osteoprotegerin and receptor activator of nuclear factor-B ligand expression in MG-63 osteoblastlike cells exposed to polyethylene particles"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : Effects of alpha-calcitonin gene-related peptide on osteoprotegerin and receptor activator of nuclear factor- B ligand expression in MG-63 osteoblastlike cells exposed to polyethylene particles | Xu et al. Journal of Orthopaedic Surgery and Research 2010 5 83 http www.josr-online.eom content 5 1 83 JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH RESEARCH ARTICLE Open Access Effects of alpha-calcitonin gene-related peptide on osteoprotegerin and receptor activator of nuclear factor-vB ligand expression in MG-63 osteoblastlike cells exposed to polyethylene particles 1 2i 1 3 i 1 1 Jie XU Max D Kauther Julia Hartl Christian vvedemeyer Study was performed at the University of Duisburg - Essen Germany Abstract Background Recent studies demonstrated an impact of the nervous system on particle-induced osteolysis the major cause of aseptic loosening of joint replacements. Methods In this study of MG-63 osteoblast-like cells we analyzed the influence of ultra-high molecular weight polyethylene UHMWPE particles and the neurotransmitter alpha-calcitonin gene-related peptide CGRP on the osteoprotegerin receptor activator of nuclear factor-KB ligand receptor activator of nuclear factor B OPG RANKL RANK system. MG-63 cells were stimulated by different UHMWPE particle concentrations 1 100 1 500 and different doses of alpha-CGRP 10-7 M 10-9 M 10-11 M . RANKL and OPG mRNA expression and protein levels were measured by RT-PCR and Western blot. Results Increasing particle concentrations caused an up-regulation of RANKL after 72 hours. Alpha-CGRP showed a dose-independent depressive effect on particle-induced expression of RANKL mRNA in both cell-particle ratios. RANKL gene transcripts were significantly P 0.05 decreased by alpha-CGRP treatment after 48 and 72 hours. OPG mRNA was significantly down-regulated in a cell-particle ratio of 1 500 after 72 hours. Alpha-CGRP concentrations of 10-7 M lead to an up-regulation of OPG protein. Conclusion In conclusion a possible osteoprotective influence of the neurotransmitter alpha-CGRP on particle stimulated osteoblast-like cells could be shown. Alpha-CGRP might be important for bone metabolism under conditions of particle-induced .