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Báo cáo khoa học: Functional analysis of two divalent metal-dependent regulatory genes dmdR1 and dmdR2 in Streptomyces coelicolor and proteome changes in deletion mutants

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In Gram-positive bacteria, the expression of iron-regulated genes is medi-ated by a class of divalent metal-dependent regulatory (DmdR) proteins. We cloned and characterized twodmdR genes of Streptomyces coelicolor that were located in two different nonoverlapping cosmids. Functional ana-lysis of dmdR1anddmdR2was performed by deletion of each copy. Dele-tion of dmdR1resulted in the derepression of at least eight proteins and in the repression of three others, as shown by 2D proteome analysis.

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