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Lecture Microbiology - Chapter 8: Gene transfer and genetic engineering

Lecture Microbiology - Chapter 8: Gene transfer and genetic engineering. This chapter presents the following content: Gene transfer, transformation - history, transformation, transduction, conjugation, plasmid types, protoplast fusion,. | GENE TRANSFER AND GENETIC ENGINEERING CHAPTER 8 Copyright © 2012 John Wiley & Sons, Inc. All rights reserved. Gene transfer Vertical – DNA transfer to progeny – Eukaryotes - Sexual reproduction – Prokaryotes - Mutation Lateral (horizontal) – Donation of DNA across the same generation Copyright © 2012 John Wiley & Sons, Inc. All rights reserved. Transformation - History Griffith (1920s) Killed versus unkilled strains “Transforming principle” Copyright © 2012 John Wiley & Sons, Inc. All rights reserved. Transformation Mechanism – Naked DNA uptake Significance – Recombinant genotypes are created Copyright © 2012 John Wiley & Sons, Inc. All rights reserved. Transduction Mechanism – Viral particles carry bacterial DNA from one cell to another Significance – Recombinant bacteria with new genetic makeup are created Copyright © 2012 John Wiley & Sons, Inc. All rights .