Đang chuẩn bị liên kết để tải về tài liệu:
Selection of indigenous strains (Paecilomyces lilacinus) Parasitize meloidogyne spp. isolated from Ba Ria – Vung Tau province

Twenty two Paecilomyces lilacinus strains were isolated from forest soils and black pepper rhizospheres in Ba Ria –Vung Tau Province. The ability to degrade chitin of PB 3.3, PB 2.9, QT2, and QT5 strains was high. The ability to degrade casein of PB 1.3, PB 2.10, KL5, and KL6 strains was efficient. | Journal of Science and Technology 55 (1A) (2017) 45-53 DOI: 10.15625/2525-2518/55/1A/12379 SELECTION OF INDIGENOUS STRAINS (Paecilomyces lilacinus) PARASITIZE Meloidogyne spp. ISOLATED FROM BA RIA – VUNG TAU PROVINCE Nguyen Thi Thuy Duong1, *, Vo Thi Thu Oanh2, Duong Hoa Xo1 1 Biotechnology Center of Ho Chi Minh City, 2374 Highway 1, District 12, Ho Chi Minh City 2 Nong Lam University, Block 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City * Email: nguyenduongbiology@gmail.com Received: 30 October 2016; Accepted for publication: 30 May 2017 ABSTRACT Twenty two Paecilomyces lilacinus strains were isolated from forest soils and black pepper rhizospheres in Ba Ria –Vung Tau Province. The ability to degrade chitin of PB 3.3, PB 2.9, QT2, and QT5 strains was high. The ability to degrade casein of PB 1.3, PB 2.10, KL5, and KL6 strains was efficient. And then, these strains were parasitized females and egg masses of Meloidogyne spp. in vitro. In female parasitism test, the rates of parasitizing female nematodes reached more than 50 % after treating for 2 days. Four strains of PB 2.10, PB 1.3, KL6 and QT5 belonged to the first group achieved the highest parasitic (> 90 %) effects on female after 3 days of incubation. In egg masses parasitism test, three strains of PB 1.3, PB 2.10 and QT5 exhibited 83.33 %, 75 % and 75 % parasitism on egg masses after 11 days of incubation. The rates of parasitizing female were higher than egg masses. Three selected strains from the experiments were PB 1.3, PB 2.10 and QT5. Keywords: Paecilomyces lilacinus, chitinase, protease, parasitize. 1. INTRODUCTION Meloidogyne spp., root-knot nematodes, are serious pests of many crops. First, these nematodes infect the roots of plants. The created wounds on root surface are gates of infecting of soil fungi such as Fusarium spp., Phytophthora spp., Sclerotium rolfsii, and Rhizoctonia solani. It significantly reduces the yield of black pepper in Ba Ria-Vung Tau Province and other areas in .