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Decellularization of rat adipose tissue, diaphragm, and heart: a comparison of two decellularization methods

Decellularization is a process that involves the removal of cellular material from the tissues and organs while maintaining the structural, functional, and mechanical properties of extracellular matrix. | Turkish Journal of Biology http://journals.tubitak.gov.tr/biology/ Research Article Turk J Biol (2018) 42: 537-547 © TÜBİTAK doi:10.3906/biy-1807-109 Decellularization of rat adipose tissue, diaphragm, and heart: a comparison of two decellularization methods Melis SESLİ , Esin AKBAY* , Mehmet Ali ONUR Department of Biology, Faculty of Science, Hacettepe University, Ankara, Turkey Received: 13.07.2018 Accepted/Published Online: 02.11.2018 Final Version: 10.12.2018 Abstract: Decellularization is a process that involves the removal of cellular material from the tissues and organs while maintaining the structural, functional, and mechanical properties of extracellular matrix. The purpose of this study was to carry out decellularization of rat adipose tissue, diaphragm, and heart by using two different methods in order to compare their efficiency and investigate proliferation profiles of rat adipose-tissue-derived mesenchymal stem cells (AdMSCs) on these scaffolds. Tissues were treated with an optimized detergent-based decellularization (Method A) and a freeze-and-thaw-based decellularization (Method B). AdMSCs were then seeded on scaffolds having a density of 2 × 105 cells/scaffold and AO/PI double-staining and MTT assays were performed in order to determine cell viability. In this study, which is the first research comparing two methods of decellularization of an adipose tissue, diaphragm, and heart scaffolds with AdMSCs, Method A provided efficient decellularization in these three tissues and it was shown that these porous scaffolds were cyto-compatible for the cells. Method B caused severe tissue damage in diaphragm and insufficient decellularization in heart whereas it also resulted in cyto-compatible adipose tissue scaffolds. Key words: Scaffold, decellularization, extracellular matrix, rat, adipose-tissue-derived mesenchymal stem cells 1. Introduction Extracellular matrix (ECM) is a structure composed of many types of proteins and polysaccharides such .