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Effects of dosages and application periods of EDTA on morphological and cytogenetic characters of barley (Hordeum vulgare L.) seedlings

Ethylenediaminetetraacetic acid (EDTA) has many applications in plant research in addition to other applications, such as chelation and medical therapy. The morphological and cytogenetic effects of EDTA on barley seedlings were investigated in the present study. | Turk J Biol 30 (2006) 59-63 © TÜB‹TAK Effects of Dosages and Application Periods of EDTA on Morphological and Cytogenetic Characters of Barley (Hordeum vulgare L.) Seedlings Ali ‹rfan ‹LBAfi, Yasemin ERO⁄LU, Halil Erhan ERO⁄LU Department of Biology, Yozgat Faculty of Arts and Sciences, Erciyes University, 66100 Yozgat-TURKEY Received: 11.04.2005 Abstract: Ethylenediaminetetraacetic acid (EDTA) has many applications in plant research in addition to other applications, such as chelation and medical therapy. The morphological and cytogenetic effects of EDTA on barley seedlings were investigated in the present study. Seeds of Hordeum vulgare L. were treated with different doses of EDTA (30, 60, 90, 120 and 150 mmol/l) and 2 different application periods (20 and 22 h). Except mitotic index, all of the measurements for the investigated characters were carried out on days 7 and 14. EDTA treatments affected germination rates in nearly all treatments. In general, EDTA treatments decreased the root, leaf and coleoptile lengths, but it had the greatest effect on coleoptile length. Increasing the EDTA dosages and treatment periods also decreased the mitotic index compared to the untreated control. The inhibitory effects of EDTA dosages on the mitotic index indicate that EDTA can have genotoxic and mutagenic effects on barley seedlings. Key Words: EDTA, Hordeum vulgare, mitotic index Farkl› Doz ve Sürelerde Uygulanan EDTA’n›n Arpa Fidelerinin Morfolojik ve Sitogenetik Karakterleri Üzerine Etkisi Özet: Etilendiamintetraasetik asit (EDTA) bitki araflt›rmalar›nda birçok kullan›m alan›na sahip olan bir kimyasald›r. Bu çal›flmada, EDTA’n›n arpa fideleri üzerindeki morfolojik ve sitogenetik etkileri incelenmifltir. Hordeum vulgare L. tohumlar› farkl› dozlarda (30, 60, 90, 120 and 150 mmol/L) ve farkl› sürelerde (22 ve 24 saat) EDTA uygulamas›na maruz b›rak›lm›fllard›r. Mitotik indeks d›fl›nda, incelenen karakterlere ait ölçümler 7. ve 14. günde gerçeklefltirilmifltir. EDTA .