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Liver total glycogen, mitochondrial protein and CoQ10 Analysis in CoQ10 supplemented broiler chicken

A Pilot study was conducted to find out the level of CoQ10 inclusion in broiler diet to bring about better performance and conducted during the period from November to December. Fifty numbers of day old broiler straight run chicks were wing banded, weighed and randomly allotted to five groups of ten chicks each based on the body weight. The treatments include, 20mg, 40mg, 60mg, 80mg, 100mg of CoQ10/kg diet. Based on the results of FCR in pilot study experiment II was conducted with Two hundred and forty (240) numbers of day old broiler straight run chicks were wing banded, weighed and randomly allotted to 5 groups 6 replicates of eight chicks each based on the body weight. The treatments were, Basal diet without CoQ10 supplementation, Low energy diet without CoQ10 supplementation, Low energy diet with 20mg of CoQ10/kg diet, Low energy diet with 40mg of CoQ10/kg diet, Low energy diet with 60mg of CoQ10 /kg diet. Glycogen content of the liver was estimated as per the method of Seifter et al., (1949). Hepatic mitochondria were obtained by differential centrifugation as outlined by Cawthon et al., (1999). Mitochondrial protein concentration was estimated as per the method of Lowry et al., (1971). The CoQ10 content of liver was extracted as per the method of (Barros et al., 2011) and liver CoQ10 level was estimated as per the method of (Ioana et al., 2009). | Liver total glycogen, mitochondrial protein and CoQ10 Analysis in CoQ10 supplemented broiler chicken