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In-vitro evaluation of bio-control agents against alternaria leaf blight of sunflower and vascular wilt of beans

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Four fungal bio-control agents, viz., Trichoderma harzianum, T. viride, Aspergillus spp. and Tricothecium spp. were evaluated in vitro through dual culture technique against Alternaria helianthi, the causative agent of leaf blight of sunflower and Fusarium oxyporum f. sp. phaseoli, the causative agent of vascular wilt of beans. The observations were taken after fourth and seventh day for both the pathogens. After fourth day, observations recorded in case of A. helianthi, the minimum colony diameter (0 cm) and maximum inhibition percentage over control (100%) were observed in Trichoderma harzianum and T. viride which were statistically at par with Aspergillus spp. and Tricothecium spp. while after seventh day, the result was more or less similar. The minimum colony diameter (0 cm) of pathogen were observed in Trichoderma harzianum and T. viride with maximum inhibition per cent (100%) over control, which differed statistically from Aspergillus spp. Tricothecium spp. showed least effectiveness against the target pathogen showing colony diameter (2.3 cm) and inhibition (23.33%) after fourth day and colony diameter (4.5 cm) and inhibition (35.71%) after seventh day over control. After fourth day, the observations taken in case of F. oxyporum f. sp. phaseoli, the minimum colony diameter (1.6 cm) and maximum inhibition per cent over control (77.14%) were observed in T. harzianum which were statistically at par with T. viride, Aspergillus spp. and Tricothecium spp. After seventh day the result was more or less similar. The minimum colony diameter (1.2 cm) of pathogen was observed in T. harzianum with maximum inhibition percentage (86.66%) over control, which differed statistically from T. viride and Aspergillus spp. Tricothecium spp. showed least effectiveness against the target pathogen showing colony diameter (3.5 cm) and inhibition per cent (50%) after fourth day and colony diameter (3.0 cm) and inhibition per cent (66.66%) after seventh day over control. | In-vitro evaluation of bio-control agents against alternaria leaf blight of sunflower and vascular wilt of beans

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