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Percent yield of ovine mesenchymal stem cells (ADMSCs) from different sources of adipose tissue

Adipose derived mesenchymal stem cells are gaining importance due to its wide distribution and ability to differentiate into tissues of mesenchymal origin viz., bone, cartilage, muscle and adipocytes. In the present study, adipose tissue samples from subcutaneous, omental, renal and perineal fat where collected and ADMSCs were isolated using 0.075 percent of collagenase type I. Cell viability was determined by trypan blue exclusion test and the viable cells were counted and cell yield was calculated. In this study, cell yield was found to be highest in renal fat (10.50±0.22 million per ml) and lowest in scrotal fat (0.75±0.09 million per ml). Subcutaneous fat yielded 2.12±0.19 million per ml, perineal fat 2.23±0.08 million per ml and omental fat 1.59±0.18 million per ml. Though yield of ADMSCs was the maximum in renal fat, due to the easy and non-invasive collection procedure and equally high level of viability, subcutaneous fat was considered as the choice of tissue collection site for further studies in sheep. | Percent yield of ovine mesenchymal stem cells (ADMSCs) from different sources of adipose tissue