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Systemic and local humoral immune response against F-1 and lasota strains of new castle disease virus in chicken

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F-1 and LaSota strains of NDV were propagated in laboratory using 10-day old embryonated hen eggs via allantoic cavity route. One group of thirty chicks were infected with F-1strain and other with LaSota strain with 105 egg infective doses in 100 µl of virus via oral and ocular route. Sequential sera samples and tracheal samples at day 0, 3,7,14, 21 and 28 post infection were collected and titre of antibodies indicative of humoral immune response was determined by indirect ELISA. The findings of present study lead to the conclusion that infection with NDV induces production of humoral immune response in chickens. Humoral antibodies generated in responses to NDV infection in chickens are both of local (IgA, IgG) and serum antibodies (IgG and IgM) type. The F-1 strain of NDV appears to be slightly better immunogenic than that of LaSota strain of NDV. | Systemic and local humoral immune response against F-1 and lasota strains of new castle disease virus in chicken

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