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In vivo haploid induction leads to increased frequency of twin-embryo and abnormal fertilization in maize

In vivo haploid induction (HI) based on Stock6-derived inducer lines has been the most prevalent means of producing haploids. Nevertheless, the biological mechanism of HI is not fully understood, the twinembryo kernels had been found during haploid induction, which may provide potential evidence for the abnormal double fertilization during HI. | Nội dung Text In vivo haploid induction leads to increased frequency of twin-embryo and abnormal fertilization in maize .den color inherit .ttnd ol .ttnd ul .ttnd dl padding 0 0px 0 20px .ttnd hr margin 10px 0px .ttnd a href javascript void 0 .ttnd a href color inherit dtextscript p text-align left dtextscript img vertical-align middle Liu et al. BMC Plant Biology 2018 18 313 lt br gt https doi.org 10.1186 s12870-018-1422-2 lt br gt lt br gt lt br gt lt br gt lt br gt RESEARCH ARTICLE Open Access lt br gt lt br gt In vivo haploid induction leads to increased lt br gt frequency of twin-embryo and abnormal lt br gt fertilization in maize lt br gt Liwei Liu Wei Li Chenxu Liu Baojian Chen Xiaolong Tian Chen Chen Jinlong Li and Shaojiang Chen lt br gt lt br gt lt br gt Abstract lt br gt Background In vivo haploid induction HI based on Stock6-derived inducer lines has been the most prevalent lt br gt means of producing haploids. Nevertheless the biological mechanism of HI is not fully understood the twin- lt br gt embryo kernels had been found during haploid induction which may provide potential evidence for the abnormal lt br gt double fertilization during HI. lt br gt Results We investigated twin-embryo frequency in progenies of different haploid inducers. Results reveal that lt br gt increasing the HI potential significantly improved the frequency of twin-embryo kernels. Compared with the lt br gt average twin-embryo kernel frequency average frequency 0.07 among progenies pollinated by the haploid lt br gt inducer line CAUHOI the frequency of twin-embryo was improved to 0.16 in progenies pollinated by the haploid lt br gt inducer line CAU5. This result was further confirmed by pollinating single hybrid ND5598 with four haploid inducers lt br gt possessing differentiated HIRs where twin-embryo frequency was highly correlated with HIR. Among 237 twin- lt br gt embryo kernels we identified 30 haploid twin-embryo kernels 12.66 a frequency which was much greater than lt .