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Prediction, synthesis and evaluation of an antimicrobial peptide from Goat Vitronectin (Vn)

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Antimicrobial resistance is an emerging challenge for health personnel and researchers. In such scenario, alternative strategies targeting novel ways to kill or inhibit growth of microbes are need of the hour. Antimicrobial peptide can be a promising candidate to deal with this crisis. Extracellular matrix protein vitronectin (Vn) is recently being established as a bactericidal agent against certain microbes. Caprine Vn has got few structural alterations as compare to human Vn. Here we have applied bioinformatics approaches to screen as well as characterise regions of goat Vn having antimicrobial peptide followed by synthesis of a predicted peptide and evaluation of the antimicrobial effect of the same against Escherichia coli DH5-α cells. The minimum bactericidal concentration (MBC) was determined by resazurin reduction test (RRT) and found to be 250 µg /107 E. coli cells. The peptide gave visible inhibition against the test organism. | Nội dung Text Prediction synthesis and evaluation of an antimicrobial peptide from Goat Vitronectin Vn

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