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Minimization of apoptosis like changes by supplementing Z-DEVD-FMK (CASPASE inhibitor) during cryopreservation of buffalo bull semen

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Present study was conducted to evaluate the anti-apoptotic effect of caspase inhibitor (ZDEVD-FMK) supplementation in buffalo bull semen. Z-DEVD-FMK was supplemented with Tris egg yolk extender @ 2, 4, 6, 10, 20 µM. Pre-freeze and post thaw samples were evaluated in terms of % individual motility, % viability, % HOS reactive sperms, status of mitochondrial membrane potential and status of sperm membrane phosphatidylserine. There was no significant effect after supplementation of caspase inhibitor on % sperm motility, % active mitochondria and % sperms with low PLA activity in pre-freeze semen samples. Supplementation of Z-DEVD-FMK did not improve sperm motility in post freeze semen sample also. | Minimization of apoptosis like changes by supplementing Z-DEVD-FMK CASPASE inhibitor during cryopreservation of buffalo bull semen

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