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Serological, microscopical and molecular examinations of cow milk samples for diagnosis of Paratuberculosis at mhow of Madhya Pradesh in India

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Mycobacterium avium subspecies paratuberculosis (MAP) is a Gram positive, acid-fast bacterium which causes Johne’s disease or Paratuberculosis in domestic livestock species. For screening of cows for MAP infection a total of 103 milk samples from individual cows were collected from organized (57) and unorganized (46) farms. Presence of anti-MAP antibodies in milk were screened by indirect enzyme linked immunosorbent assays (iELISA) test, 72 (69.90%) cow milk samples were found positives (66.07% from organized farms and 74.46% from unorganized farms). Positivity percentages were 62.50 in Gir cows and 70.52 in non-descriptive and crossbred cows irrespective of the farming system for the presence of MAP antibodies. | Serological microscopical and molecular examinations of cow milk samples for diagnosis of Paratuberculosis at mhow of Madhya Pradesh in India